Figure 6.

Effects of IFI16 overexpression on pEF-GFP transfection efficiency (%GFP⁺ cells) in HEK-293T cells

Untransfected negative control cells were not transfected, while positive control cells were only transfected with pEF-GFP (green box). The blue box represents cells that were transfected with the luciferase expression plasmid pGL4.50 one day prior to transfection with pEF-GFP, while the red box represents cells that were transfected with the IFI16 expression plasmid pIFI16-FL one day before transfection. Horizontal lines within boxes represent the means for each group, while boxes show the interquartile range, and the entire dataset is contained within the whiskers. Letters (a, b, c) indicate samples with significantly different transfection efficiencies (n = 18 for each sample, p < 0.05 using a Wilcoxon rank-sum test).