Table 1.
Expression levels (TPM) of genes involved in DNA sensing pathways in each cell line.
| HEK-293T |
PC-3 |
Jurkat T |
Primary T |
||||||
|---|---|---|---|---|---|---|---|---|---|
| Ctrl | trans | Ctrl | trans | Ctrl | trans | Ctrl | trans | ||
| PYHIN sensors | IFI16 | 0 | 0 | 25.7 | 945.5 | 294.1 | 266.8 | 274.9 | 215.1 |
| PYHIN1 | 0 | 0 | 0 | 0.1 | 1.1 | 0.9 | 19.6 | 10.2 | |
| MNDA | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | |
| AIM2 | AIM2 | 0 | 0 | 0 | 10.0 | 0 | 0 | 28.8 | 34.1 |
| PYCARD | 0.1 | 0.1 | 6.1 | 6.8 | 0 | 0 | 63.0 | 22.9 | |
| CASP1 | 0 | 0 | 0 | 56.0 | 0 | 0 | 29.9 | 24.0 | |
| LRR-FIP1 | LRRFIP1 | 39.1 | 45.0 | 135.3 | 129.7 | 105.3 | 96.4 | 168.2 | 159.8 |
| CTNNB1 | 225.4 | 311.2 | 269.0 | 195.8 | 105.6 | 99.0 | 105.5 | 103.8 | |
| TCF4 | 5.5 | 6.9 | 10.5 | 21.2 | 2.6 | 2.3 | 0.7 | 0.5 | |
| STING-axis DNA-sensing pathway |
CGAS | 0.1 | 0.2 | 25.2 | 46.5 | 0 | 0 | 42.0 | 49.8 |
| DDX41 | 58.6 | 63.0 | 123.0 | 91.6 | 113.2 | 121.3 | 73.2 | 77.3 | |
| ZBP1 | 0.1 | 0.1 | 0 | 5.8 | 0 | 0 | 1.3 | 0.2 | |
| POLR3A | 54.9 | 52.9 | 97.5 | 107.3 | 30.0 | 29.0 | 18.0 | 19.1 | |
| DDX58 | 2.7 | 2.3 | 17.8 | 541.2 | 11.3 | 10.1 | 9.6 | 7.0 | |
| MAVS | 13.9 | 18.2 | 46.0 | 23.6 | 27.0 | 26.4 | 30.7 | 25.0 | |
| STING1 | 0.2 | 0.6 | 19.0 | 42.2 | 56.6 | 59.9 | 144.1 | 141.7 | |
| TBK1 | 44.1 | 38.1 | 40.8 | 39.9 | 30.8 | 25.7 | 54.0 | 51.7 | |
| MYD88-axis DNA-sensing pathway |
TLR9 | 0.1 | 0.1 | 0.1 | 0.4 | 0 | 0 | 0.9 | 0.4 |
| DHX9 | 260.9 | 288.0 | 133.0 | 85.6 | 341.0 | 311.8 | 198.7 | 222.8 | |
| DHX36 | 63.8 | 65.3 | 101.1 | 94.3 | 88.8 | 73.9 | 51.5 | 53.0 | |
| MYD88 | 1.3 | 1.9 | 52.9 | 298.3 | 77.8 | 84.3 | 60.9 | 48.4 | |
| IRAK1 | 171.5 | 195.1 | 163.9 | 147.2 | 158.5 | 162.0 | 113.1 | 135.7 | |
| IRAK4 | 8.9 | 8.5 | 13.4 | 14.3 | 24.0 | 21.1 | 18.1 | 14.9 | |
| TRAF6 | 4.0 | 4.8 | 8.0 | 9.6 | 7.1 | 6.8 | 6.0 | 6.1 | |
| MAP3K7 | 34.6 | 36.1 | 37.3 | 22.1 | 51.8 | 44.8 | 29.7 | 26.8 | |
| TAB1 | 36.7 | 40.3 | 18.2 | 22.4 | 26.4 | 28.7 | 21.9 | 17.0 | |
| TAB2 | 63.5 | 65.7 | 47.4 | 61.0 | 33.8 | 30.0 | 117.7 | 113.2 | |
| MAP2K4 | 34.8 | 40.6 | 28.4 | 18.5 | 26.4 | 24.5 | 24.4 | 26.8 | |
| MAP2K7 | 54.9 | 54.3 | 36.6 | 41.3 | 39.4 | 42.2 | 39.6 | 38.3 | |
| MAPK8 | 20.2 | 20.8 | 21.5 | 18.1 | 102.1 | 89.7 | 22.4 | 24.1 | |
| MAPK9 | 57.4 | 55.3 | 39.9 | 21.8 | 47.1 | 44.1 | 29.3 | 27.2 | |
| MAP2K3 | 31.6 | 39.7 | 46.6 | 74.4 | 53.6 | 55.1 | 178.0 | 170.9 | |
| MAP2K6 | 6.8 | 14.9 | 41.5 | 19.4 | 17.0 | 14.6 | 4.5 | 1.8 | |
| MAPK14 | 42.2 | 40.7 | 44.3 | 28.0 | 83.5 | 77.7 | 41.7 | 34.7 | |
| JUN | 104.4 | 154.1 | 47.6 | 159.7 | 6.4 | 5.7 | 144.4 | 138.7 | |
| MAP3K14 | 6.5 | 5.5 | 14.4 | 25.3 | 5.0 | 5.5 | 38.6 | 34.3 | |
| IKBKE | 0.4 | 0.5 | 9.7 | 18.6 | 18.9 | 20.0 | 16.0 | 9.0 | |
| NFκBIA | 16.9 | 18.8 | 67.5 | 431.7 | 32.2 | 31.5 | 394.7 | 525.5 | |
| Transc. factors | NF-κB | 18.7 | 20.9 | 34.7 | 44.0 | 52.5 | 48.2 | 97.5 | 103.9 |
| IRF5 | 0 | 0 | 2.9 | 5.4 | 0.6 | 0.6 | 36.1 | 30.1 | |
| IRF7 | 0.8 | 1.1 | 23.7 | 379.1 | 5.7 | 6.1 | 26.1 | 27.4 | |
| IRF3 | 65.5 | 71.5 | 63.5 | 79.8 | 76.3 | 81.5 | 92.2 | 82.2 | |