Table 2.
Genes expressed at significantly lower levels in electroporated PC-3 cells vs. Lipofected PC-3 cells
| Vehicle: |
Lipofectamine _ |
Electroporation |
||||||
|---|---|---|---|---|---|---|---|---|
| Media: |
SFM |
SCM |
SCM |
|||||
| Symbol | Ctrl | trans | Ctrl | trans | Ctrl | trans | Padj | |
| DNA sensors and downstream pathways | IFI16 | 25.7 | 945.5 | 14.1 | 421.4 | 12.3 | 149.3 | a |
| STING1 | 19.0 | 42.2 | 20.1 | 54.1 | 8.9 | 9.1 | a | |
| JAK2 | 10.8 | 50.3 | 13.0 | 19.3 | 10.5 | 11.7 | a | |
| STAT2 | 33.8 | 261.9 | 41.0 | 210.7 | 16.3 | 52.8 | a | |
| AIM2 | 0 | 10.0 | 0 | 6.6 | 0 | 2.2 | b | |
| CASP1 | 0 | 56.0 | 0.5 | 21.8 | 0.6 | 3.8 | a | |
| Midkine, chemokines, and cytokines | MDK | 599.6 | 2058.9 | 543.2 | 990.9 | 330.5 | 332.2 | b |
| LGALS9 | 0.07 | 112.23 | 0.08 | 59.30 | 0.21 | 11.36 | a | |
| CXCL11 | 0.1 | 231.5 | 0 | 54.2 | 1.0 | 47.3 | a | |
| CXCL10 | 0.1 | 137.2 | 0 | 48.7 | 0.3 | 29.9 | a | |
| IFNL1 | 0 | 131.1 | 0 | 33.8 | 0.2 | 26.7 | a | |
| IFNB1 | 0 | 113.1 | 0 | 21.0 | 0 | 16.3 | a | |
| IFNL2 | 0 | 67.8 | 0.1 | 22.1 | 0.1 | 7.0 | b | |
| IFNL3 | 0 | 41.2 | 0 | 16.6 | 0.2 | 5.3 | b | |
| IFNA7 | 0 | 3.7 | 0 | 0.4 | 0 | 0 | b | |
| IFNA10 | 0 | 1.5 | 0 | 0.1 | 0 | 0 | b | |
| IFNA16 | 0 | 0.8 | 0 | 0.2 | 0 | 0 | b | |
| Selected CSGs | BST2 | 3.1 | 1145.8 | 0.6 | 652.8 | 0.1 | 136.3 | a |
| PSMB9 | 5.8 | 191.7 | 7.8 | 196.3 | 4.8 | 39.6 | a | |
| IFIT2 | 23.1 | 3070.6 | 44.4 | 1916.0 | 26.4 | 347.8 | a | |
| TRIM22 | 0.77 | 218.36 | 0.10 | 105.61 | 0.06 | 17.53 | a | |
| RSAD2 | 2.9 | 1418.0 | 0.9 | 768.7 | 1.3 | 185.6 | a | |
| LGALS3BP | 84.7 | 1313.9 | 163.5 | 1512.9 | 94.9 | 336.8 | a | |
| SERPINE1 | 90.4 | 789.8 | 285.9 | 1854.8 | 164.0 | 134.0 | a | |
| CD68 | 50.6 | 727.2 | 88.5 | 439.1 | 55.5 | 109.4 | a | |
| WARS1 | 98.8 | 704.1 | 119.5 | 256.3 | 99.1 | 129.1 | a | |
| SAMD9 | 12.1 | 665.7 | 14.5 | 347.7 | 11.8 | 149.2 | a | |
a
Gene expression levels significantly lower in electroporated PC-3 cells versus cells lipofected in SFM and SCM.
b
Gene expression levels significantly lower in electroporated PC-3 cells only compared to cells Lipofected in SFM (i.e., not significantly lower than PC-3 cells lipofected in SCM).