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. 2022 Dec 1;28(12):2633–2645. doi: 10.1038/s41591-022-02059-9

Extended Data Fig. 1. Viability of C-28/I2 cells upon LNA043 treatment and absence of a proliferative effect of LNA043 during chondrogenic rescue in UE7T-13 cells.

Extended Data Fig. 1

a, Viability of C-28/I2 cells upon LNA043 treatment. Absence of cytotoxicity of LNA043 in C-28/I2 cells treated for 24 h in C-28/I2 test medium supplemented with LNA043 was determined by CellTiter-Glo® (Promega). Levels are expressed in relative light units (RLU). Values are mean ± s.d. of measurements from a representative experiment with biological triplicates. b, Absence of a proliferative effect of LNA043 during the chondrogenic rescue in UE7T-13 cells. Proliferation was assessed in UE7T-13 cells cultivated for 3, 7, 11 and 14 d in UE7T-13 chondrogenic medium supplemented or not with 0.5 μg ml-1 Poly I:C (PIC), 100 or 200 μg ml-1 LNA043 by CellTiter-Glo® (Promega). Levels are expressed in relative light units (RLU). Values are mean ± s.d. of measurements from biological triplicates. In a and b statistical significance was determined by one-way ANOVA. No significant differences were observed between any of the conditions.