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. 2022 Nov 22;299(1):102731. doi: 10.1016/j.jbc.2022.102731

Figure 1.

Figure 1

Total zinc content increases during follicle development via active accrual.A, illustration and bright field images of ovarian primordial, primary, and secondary stage follicles. The scale bar represents 20 μm. B, elemental maps of iron, copper, and zinc for each follicular stage established using synchrotron-based X-ray fluorescence microscopy. The color scale bar represents the minimum and maximum elemental contents (μg/cm2) of each element. The scale bar represents 10 μm. C, quantification of total atom number of iron, copper, and zinc from the elemental maps, with whole follicle area as the region of interest (ROI). D, quantification of elemental concentration in μg/cm2 of iron, copper, and zinc from the elemental maps. E, total zinc atom per follicle per hour uptake in the primordial and growing follicles of primary and secondary follicle stages measured using a Zn-65 uptake method. Data represent mean values (SD). N= 10 primordial follicles, 8 primary follicles, 6 secondary follicles (B–D), and 3 independent repeats of Zn-65 uptake experiments on pools of 100 to 500 primordial follicles and 200 growing follicles (E). Letters denote statistically significant differences between developmental stages for each element by two-way ANOVA with Tukey’s multiple comparison test (C and D, p < 0.05) (C: Secondary to primary stage total iron Padj = 0.0004, total zinc Padj < 0.0001) (D: Secondary to primary stage zinc concentration Padj < 0.0001) or unpaired t test (E). See Table S1 for corresponding quantification.