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. 2001 Feb;69(2):1192–1198. doi: 10.1128/IAI.69.2.1192-1198.2001

FIG. 3.

FIG. 3

Amplification of full-length and truncated versions of ssp-2. The wild-type ssp-2 was amplified from DNA of P. falciparum clone 3D7 of strain NF54 using primers 1 and 2 and cloned into pBluescript II KS. This cloned plasmid DNA was thereafter used as the template for deriving different versions of ssp-2 that were amplified by PCR and cloned into the Nsil site of pMOhly1. Oligonucleotide primers 20 and 23 amplified the wild-type ssp-2, primers 20 and 27 amplified ssp-2 without the C terminus, primers 21 and 23 amplified ssp-2 without the N terminus, and the primers 30 and 27 amplified SSP-2 without the N and C termini. Summarizing data from Fig. 1 and 4, expression refers to the detection of SSP-2 in whole-cell lysates by immunoblotting with monoclonal antibody SSP-2.1; secretion results in detection of SSP-2 in immunoblots of culture supernatants. LP, leader peptide; TM, transmembrane domain.