Skip to main content
. 2001 Feb;69(2):1192–1198. doi: 10.1128/IAI.69.2.1192-1198.2001

FIG. 4.

FIG. 4

Expression and secretion of SSP-2 by the hemolysin secretion system detected by immunoblot analysis of culture supernatants using monoclonal antibody SSP-2.1. (A) Culture supernatants of serovar Typhimurium SL3261 carrying pSK and pMOhly1 (pMO) constructs encoding full-length SSP-2 and truncated versions without the transmembrane α-helix domains. (B) Culture supernatants of CVD 908-htrA carrying pSK and pMOhly1 encoding full-length SSP-2 and truncated versions without transmembrane α-helix domains. M, molecular weight markers. Control lane (+) contains the bacterial lysate from E. coli DH5α(pSK-2023), which expresses full length SSP-2.