Table 1.
Strains and plasmids used in this study.
| Strain and plasmid | Charactersa | Reference or source |
|---|---|---|
| Strains | ||
| Escherichia coli | ||
| DH5α | endA1 hsdR17 supE44 thi-1 λ− recA1 gyrA96 relA1 ΔlacU169(Φ80dlacZΔM15) | TakaRa |
| EC135 | mcrA Δ(mrr-hsdRMS-mcrBC) Δdcm::FRT Δdam::FRT | Zhang et al. (2012) |
| Bacillus velezensis | ||
| PG12 | Wild-type strain isolated from apple | Chen et al. (2016a) |
| ΔdgcK | ΔdgcK::ErmR, | Yang et al. (2018) |
| ΔdgcP | ΔdgcP::ErmR | Yang et al. (2018) |
| ΔpdeH | ΔpdeH::TetR | Yang et al. (2018) |
| ΔydaK | ΔydaK::ErmR | Yang et al. (2018) |
| ΔykuI | ykuIΔEAL::TetR | Yang et al. (2018) |
| ΔmotI | ΔmotI::ErmR | This study |
| ΔdgcPydaK | ΔdgcP::ErmRydaK::TetR | This study |
| PG12(pUBXC) | pUBXC in PG12, ZeoR | Yang et al. (2018) |
| PG12(pUBX) | pUBX in PG12, ZeoR | Yang et al. (2018) |
| ΔdgcP (pUBX) | pUBX in ΔdgcP, ErmR,ZeoR | Yang et al. (2018) |
| ΔydaK(pUBX) | pUBX in ΔydaK, ErmR,ZeoR | This study |
| ΔydaK(pUBX-ydaK) | pUBX-ydaK in ΔydaK, ErmR,ZeoR | This study |
| PG12(pUBX-pdeH) | pUBX-pdeH in PG12, ZeoR | This study |
| ΔdgcP(pUBX-pdeH) | pUBX-pdeH in ΔdgcP, ErmR,ZeoR | This study |
| ΔpdeH(pUBX-dgcP) | pUBX-dgcP in ΔpdeH, TetR,ZeoR | Yang et al. (2018) |
| ΔydaK(pUBX-pdeH) | pUBX-dgcP in ΔydaK, ErmR,ZeoR | This study |
| ΔykuI (pUBX-pdeH) | pUBX-dgcP in ΔykuI, TetR,ZeoR | This study |
| ΔmotI(pUBX-pdeH) | pUBX-dgcP in ΔmotI, ErmR,ZeoR | This study |
| ΔdgcPydaK(pUBX-pdeH) | pUBX-dgcP in ΔdgcPydaK, TetR,ErmR,ZeoR | This study |
| Plasmids | ||
| pWYE782 | gp35 cloned into pAX01, EmR | Sun et al. (2015) |
| pMD19-T | TA cloning vector, AmpR | TakaRa, Dalian, China |
| pGFP78 | pHY300PLK containing GFP gene, AmpR and TetR | Zhang et al. (2017) |
| pMDT3 | pMD19-T containing the TetR gene from pGFP78, AmpR and TetR | Fan et al. (2017) |
| pMDE-motI | pMDE containing motI upper and lower arm fragment, ErmR, AmpR | This study |
| pMDT3-ydaK | pMDT3 containing ydaK upper and lower arm fragment, TetR, AmpR | Yang et al. (2018) |
| pUBX | Bacillus-Escherichia coli shuttle vector containing PxylA, xylR, t0 transcription terminator, ZeoR | Chen et al. (2016b) |
| pUBXC | pUBX containing comKBsu under the control of xylose inducible system, ZeoR | Chen et al. (2016b) |
| pUBX-pdeH | pUBX containing pdeH under the control of xylose inducible system, ZeoR | Yang et al. (2018) |
| pUBX-dgcP | pUBX containing dgcP under the control of xylose inducible system, ZeoR | Yang et al. (2018) |
| pUBX-ydaK | pUBX containing ydaKPG12 under the control of xylose inducible system, ZeoR | This study |
aErmR, TetR, ZeoR, AmpR stand for resistance to erythromycin, tetracycline, zeocine and ampicillin, respectively.