Table 3.
Effects of BY-kinase mutations on observed phenotype
| BY-kinase | Mutation | Kinase activity | Phenotype | Proposed to stabilize | Reference |
|---|---|---|---|---|---|
| V. cholerae VpsO | K551A (Walker-A) | Abolished | Rougher colonies, higher EPS levels | ΣΟ | Schwechheimer (13) |
| VpsU C12S (inactive cognate LMW-PTP) | — | Smooth colonies, lower EPS levels | ΣΜ | ||
| E519A/R522A/R525A (O-motif) | Reduced | Smooth colonies, lower EPS levels | ΣΜ | ||
| Y720F/Y721F/Y726F/Y727F (Y-cluster) | — | Smooth colonies, lower EPS levels | ΣO | ||
| E. coli K-12 Wzc | Y708E/Y710E/Y11E/Y713E/Y715E (Y-cluster) | — | No EPS | ΣΜ | Obadia (53) |
| ΔY-cluster | — | No EPS | ΣΜ | ||
| K540M (Walker-A) | Abolished | No EPS | ΣΟ | ||
| Y708F/Y710F/Y11F/Y713F/Y715F (Y-cluster) | — | Uniform EPS size distribution ∼1350 kDa (600–2000 kDa in WT) | ΣΟ | ||
| Y569F | Reduced | Uniform EPS size distribution ∼1350 kDa (600–2000 kDa in WT) | ΣΟ | ||
| S. pneumoniae CpsD | Y215F/Y218F/Y221F (Y-cluster) | — | Decreased levels of LMW EPS, no effect on total abundance | ΣΟ | Nakamoto (15) |
| Y215E/Y218E/Y221E (Y-cluster) | — | Increased levels of LMW EPS, no effect on total abundance | ΣΜ | ||
| Acinetobacter baumannii Wzc | Y712F/Y714F/Y716F/Y718F/Y720F/Y722F (Y-cluster) | — | Increased levels of high molecular weight (HMW) CPS | ΣΟ | Geisinger (54) |
| D569N (Walker-A′) | Reduced | Increased levels of HMW CPS | ΣΟ | ||
| K547Q (Walker-A) | Not affected | Increased levels of HMW CPS | ΣΟ | ||
| D649N (Walker-B) | Not affected | Increased levels of HMW CPS | ΣΟ | ||
| Burkholderia cepacia BceF | ΔBceD (deletion of cognate LMW-PTP) | — | Reduced viscosity suggestive of LMW EPS | ΣΜ | Ferreira (55) |
| Acinetobacter lwoffii Wzc | Y712F/Y714F/Y716F/Y718F/Y720F (Y-cluster) | — | Increased mucoidy, enhanced levels of higher MW EPS | ΣΟ | Nakar (56) |
| B. subtilis EpsB | Y225F/Y227F (Y-cluster) | — | Hyperwrinkled colonies | ΣΟ | Esholz (57) |
| Y225E/Y227E (Y-cluster) | — | Flat colonies | ΣΜ |