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. 2022 Dec 17;8(12):e12385. doi: 10.1016/j.heliyon.2022.e12385

Figure 1.

Figure 1

The effect of high glucose on the expression levels of LOX-1, Beclin-1, and LC3II in HLSECs transfected by recombinant LV-LOX-1. HLSECs were treated with high glucose (HG), HG + recombinant lentivirus LOX-1 (LV-LOX-1), and HG + control lentiviral cells (LV-CON) for 72 h. (A–C) The mRNA expression levels of LOX-1, Beclin-1, and LC3II in HLSECs within 72 h after treatment with HG (25 mM), LV-LOX-1, and LV-CON. (D) The protein expression levels of each group were detected using western blotting (see original blots in Supplemental Figure 1D). (E–G) Western blotting analysis of the protein expression levels of LOX-1, Beclin-1, and LC3II in HLSECs within 72 h after treatment with HG and/or LV-LOX-1 and LV-CON. Western blot and qRT-PCR analyses were used to normalize the levels of GAPDH and β-actin (n = 3 independent experiments). All results are expressed as mean ± SD. ∗∗∗P < 0.001 versus the NC group; ###P < 0.001 versus HG + LV-CON group.