TABLE 1.

Effects of gingipain RgpA on leaky site formation and clearance of FITC-dextran from in situ hamster cheek pouch^a

Intervention mixture	Leaky sites (no. per 0.11 cm2)	Clearance (ml/min, 10−6)
Saline (control)	Nil	15 ± 2
GRgpA (0.001 μM)	3 ± 1*	23 ± 3*
GRgpA (0.001 μM) and NPC 17647 (1 μM)	Nil†	15 ± 4†
GRgpA (0.01 μM)	8 ± 1*	33 ± 4*
GRgpA (0.01 μM) and NPC 17647	2 ± 1†	13 ± 3†
GRgpA (0.001 μM) and leupeptin (1 μM)	Nil†	13 ± 3†
GRgpA (0.01 μM) and leupeptin (1 μM)	1 ± 1†	13 ± 2†
GRgpA (0.001 μM) and PI mixture	Nil†	13 ± 4†
GRgpA (0.01 μM) and PI mixture	1 ± 1†	18 ± 7†
GRgpA (0.001 μM) and indomethacin (10 mg/ml)	6 ± 1†	42 ± 6†
GRgpA (0.01 μM) and indomethacin (10 mg/ml)	9 ± 1†	39 ± 5†

^aValues are means ± standard errors of the means; for each group, n = 4 animals. GRgpA, gingipain RgpA; PI mixture, mixture of proteinase inhibitors consisting of aprotinin (5 μg/ml), soybean trypsin inhibitor (100 μg/ml), bestatin (10 μM), and dl-2-mercapto-methyl-3-guanidinoethylthiopropanoic acid (10 μM). An asterisk indicates a P value of <0.05 in comparison to saline (control). A dagger represents a P value of <0.05 in comparison to gingipain RgpA alone. Nil, no leaky sites were detected.