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. 2022 Dec 9;67:101652. doi: 10.1016/j.molmet.2022.101652

Figure 5.

Figure 5

p21 gives rise to hallmarks of cellular senescence. (A) Oxygen consumption rates (OCR) in muscle fiber mitochondria measured using high-resolution respirometry (n = 7/group). OCR, oxygen consumption rates; ST1, respiratory state 1; MPGS, malate, pyruvate, glutamate, succinate; ADP, respiration in presence of MPGS plus ADP; Max, maximal respiration (after FCCP titration); Rot, respiration after rotenone (complex I inhibitor) addition. (B) Representative immunofluorescence images of soleus muscle cross sections stained for γH2AX, DAPI, and dystrophin. Nuclei inside the dystrophin border were classified as myonuclei (yellow arrow) and those outside the fiber border were classified as interstitial nuclei (white arrow). (C) Quantification of the percentage of γH2AX + myonuclei and γH2AX + interstitial nuclei (n = 5/group). (D) Protein concentrations of circulating SASP factors measured with Magpix (n = 8–12/group) and ELLA (4–5/group) multiplexing platforms. Scale bar, 20 μm. Data represent means ± SD. ∗P < 0.05; ∗∗P < 0.01; ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001, as assessed by unpaired two-tailed t tests.