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. 2023 Jan 1;13(1):77–94. doi: 10.7150/thno.77404

Figure 6.

Figure 6

miR-182 is not required for the normal hematopoiesis. (A-C) The total numbers of WBC, LYM, NEU, MON, RBC, and PLT were counted by automatic blood cell counter in 182WT (n = 17) and 182KO (n = 16) mice. (D) The total numbers of BM mononuclear cells were counted in 182WT (n = 6) and 182KO (n = 6) mice. (E) T (CD3+), B (B220+), and myeloid cells (Mac-1+, Gr-1+) frequencies were performed by flow cytometry in PB from 182WT (n = 4) versus 182KO (n = 4) mice. (F) The percentages of long-term hematopoietic stem cell (LT-HSC, Lin-c-Kit+Sca-1+CD34-CD135-), short-term hematopoietic stem cell (ST-HSC, Lin-c-Kit+Sca-1+CD34+CD135-), and multipotential progenitor (MPP, Lin-c-Kit+Sca-1+CD34+CD135+) were measured by flow cytometry in 182WT (n = 4) and 182KO (n = 4) mice. (G-J) BM LSK (Lin-c-Kit+Sca-1+) cells were sorted from 182WT (n = 3) and 182KO (n = 3) mice and plated on methylcellulose medium for colony number. Shown are the representative pictures (G) and statistical analysis of colony number (H), total number (I), and the classification of burst-forming unit-erythroid (BFU-E), colony-forming unit-granulocyte, erythroid, macrophage, and megakaryocyte (CFU-GEMM), colony-forming unit-granulocyte (CFU-G), colony-forming unit-macrophage (CFU-M), and colony-forming unit-granulocyte and macrophage (CFU-GM) (J). Bar scales represent 100 μm. (K) In vivo competition assays for CD45.2/CD45.2+CD45.1 after transplantation for the indicated months. Shown are the representative plots (left) and statistical analysis of CD45.2/CD45.2+CD45.1 (right). N.S: not significant.