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. 2023 Jan 1;13(1):417–437. doi: 10.7150/thno.77694

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WWP1 expression is increased in cardiomyocytes in response to myocardial infarction. A Immunoblot analysis of WWP1 (n = 6, 14) and Bax (n = 3, 4) expression in the infarct areas (infarct and border zone) of wild type (WT) mice at day 1 post-MI as well as in the sham control. Corresponding statistics of WWP1 and Bax were shown. B Immunoblot analysis of WWP1 (n = 6, 14) and Bax (n = 3, 4) expressions in non-infarct areas at day 1 post-MI as well as in the sham control. Corresponding statistics of WWP1 and Bax were shown. C Immunohistochemistry for WWP1 (brown) in the sham heart or infarcted heart tissue. n = 4. D Immunofluorescence co-staining for cTnI with WWP1 and DAPI in the heart post-MI. n = 4. E Neonatal rat cardiac myocytes (NRCMs) were treated by hypoxia for 6h. WWP1 and Bcl2 expressions were examined by Western blots. Corresponding statistics of WWP1 and Bcl2 were shown. n = 3. The data are shown as the means ± SD. The data shown in A, B, and E were analysed by unpaired Student's t-test.