Figure 7.

Inhibition of WWP1 expression in cardiomyocytes restrains NF-κB and MAPK activation involving degrading KLF15 in the infarcted myocardium. Mice were treated with rAAV9-cTnT-shScramble or rAAV9-cTnT-shWWP1 by intravenous injection of tail two weeks before suffered from sham or MI surgery, and after additional one day, mice were sacrificed. A Ubiquitination of KLF15 was detected by immunoprecipitation with anti-KLF15 antibody followed by immunoblot with anti-Ub-K48 antibody. IgG as a negative control. n = 3. B Immunohistochemistry for KLF15 (brown) in sham or infarcted hearts. Scale bar = 50 μm. n = 3. C Immunofluorescence co-staining for cTnI with KLF15 and DAPI in mice hearts post-MI. Scale bar = 40 μm. n = 3. D-F Protein expression of KLF15 in cytoplasm and nuclear lysates harvested from infarct areas post-MI was detected by Western blots. Corresponding statistics of KLF15 were shown. n = 3. G Western blot analysis and statistical results of expression of P65-AcK310 in nucleus. n = 3. H The levels of phosphorylated P38 and ERK1/2 were examined by Western blots, and statistical results were shown. n = 3. The data are shown as the means ± SD. The data shown in E-H were analysed by one-way ANOVA followed by Bonferroni post hoc test.