Figure 9.

I3C pre-treatment suppresses WWP1-mediated excessive myocardial inflammation post-MI. Mice were administered with vehicle or I3C (20 mg/kg) three times a week for a month. When mice were pre-treated by I3C for two weeks, they were randomly assigned to rAAV9-cTnT-WWP1 or rAAV9-cTnT-GFP injection, and all of which were subjected to LAD ligation after two weeks of virus injection. All mice sacrificed 1day after induction of MI. A-E mRNA levels of IL-6, IL-1β, TNF-α, VCAM-1, and MCP-1 in infarcted or healthy hearts were tested by qRT-PCR. n = 4. F Representative Western blot and statistical result of Ly6G and KLF15 in the infarct zone. n = 3. G Western blot analysis and statistical results of expression of P65-AcK310 in nucleus. n = 3. H-J H9C2 cells were infected with Adv-WWP1 or Adv-GFP for 24 h followed by I3C (50 μM) treatment for 24 h, and then the cells were treated with hypoxia for 6 h. mRNA levels of IL-6, IL-1β, TNF-α were tested by qRT-PCR. n = 3. K Western blot analysis and statistical results of expression of P65-AcK310 in nucleus in H9C2 cells. n = 3. The data are shown as the means ± SD. The data shown in A-G were analysed by two-way ANOVA followed by Bonferroni post hoc test. H-K were analysed by one-way ANOVA followed by Bonferroni post hoc test.