Skip to main content
. 2023 Jan 1;13(1):231–249. doi: 10.7150/thno.78878

Figure 4.

Figure 4

Myeloid-specific SIRT6 knockout aggravates periodontitis and impairs inflammation resolution. (A) Phagocytic index was assessed in macrophages of myeloid-specific SIRT6 deficiency mice. (B) Representative 3D micro-CT scanning images and reconstructed sections in LIP and LIP resolution of WT and mS6KO mice. Arrowhead: the area of loss of alveolar bone. (C) Representative images of TRAP-stained paraffin sections in the periodontium of WT and mS6KO mice. (D) Representative images of TUNEL staining in the periodontium of WT and mS6KO mice. (E) Representative images of Ly6g positive neutrophils in the periodontium of WT and mS6KO mice. (F) Representative images of Cit-H3 (red) and MPO (green) positive NETs in the periodontium of WT and mS6KO mice. (G-H) Representative images of M1 and M2 in the periodontium of WT and mS6KO mice. (I-Q) Quantification of the distance of CEJ-ABC, OC. N/ BS and OC. S/BS, TUNEL positive apoptotic cells, neutrophils, NET, macrophage infiltration, and M1 and M2 polarization (n = 5 mice in the WT group and n = 5 mice in the mS6KO groups). The results were presented as means ± S.D. *p < 0.05; **p < 0.01; ***p > 0.001 by 2-tailed, unpaired Student's t test. T: Tooth, B: Bone of Alveolar, 14DL: 14 days ligated, 7DL-7DR: 7 days ligated and 7 days with ligatures removed, mS6KO: LysM-Cre+ SIRT6flox/flox. The white dotted line indicates the boundary between the root and the alveolar bone and the gingiva.