Skip to main content
. 2023 Jan 1;13(1):231–249. doi: 10.7150/thno.78878

Figure 7.

Figure 7

miR-216a-5p-216b-5p-217 cluster negatively regulates macrophages efferocytosis by targeting DEL-1 and CD36. (A) Venn diagram showing for miR-217-5p and miR-216a-5p and miR-216b-5p targeted genes predicted by miRwalk. (B) Dual-Luciferase Reporter of DEL-1and CD36, and luciferase activity in 293T cells co-transfected with miR-217-5p and miR-216a-5p and miR-216b-5p mimic. (C) Western blotting showed the expression of CD36 and DEL-1 following transfected with miR-217-5p and miR-216a-5p and miR-216b-5p mimic and inhibitors. (D) The expression of DEL-1 in diabetic periodontitis was decreased compared with periodontitis. (E) Phagocytic index of macrophages following exposure to the miR-217-5p and miR-216a-5p and miR-216b-5p inhibitor. (F) Under high glucose conditions, exogenous protein DEL-1, miR-217-5p inhibitor and overexpress SIRT6 could restore macrophage efferocytosis. (G) Schematic showing the mechanism that SIRT6 mediated macrophage efferocytosis. The results were presented as means ± S.D. *p < 0.05; **p < 0.01 by ANOVA or Student's t test.