Table 3.
Genetic diversity parameters of 159 polymorphic SSR markers used in the present study.
S. No. | Marker ID | Annealing temperature (°C) | Total amplified bands (TB) | Polymorphic bands (PB) | Percentage of polymorphic bands (PPB) | Major allele frequency | PIC value | Gene diversity |
---|---|---|---|---|---|---|---|---|
1 | BRMS-002 | 51 | 3 | 1 | 33.33 | 0.33 | 0.59 | 0.67 |
2 | BRMS-003 | 55 | 3 | 1 | 33.33 | 0.34 | 0.59 | 0.66 |
3 | BRMS-005 | 50 | 3 | 1 | 33.33 | 0.63 | 0.37 | 0.47 |
4 | BRMS-006 | 49 | 3 | 1 | 33.33 | 0.34 | 0.59 | 0.66 |
5 | BRMS-007 | 53 | 4 | 4 | 100.00 | 0.56 | 0.49 | 0.56 |
6 | BRMS-011 | 50 | 6 | 6 | 100.00 | 0.70 | 0.40 | 0.45 |
7 | BRMS-015 | 56 | 3 | 1 | 33.33 | 0.52 | 0.46 | 0.54 |
8 | BRMS-017 | 56 | 4 | 4 | 100.00 | 0.66 | 0.38 | 0.45 |
9 | BRMS-027 | 50 | 5 | 5 | 100.00 | 0.65 | 0.44 | 0.49 |
10 | BRMS-029 | 55 | 2 | 1 | 50.00 | 0.50 | 0.38 | 0.50 |
11 | BRMS-030 | 57 | 7 | 7 | 100.00 | 0.74 | 0.37 | 0.41 |
12 | BRMS-033 | 53 | 4 | 4 | 100.00 | 0.66 | 0.40 | 0.46 |
13 | Ra1-F03 | 53 | 3 | 2 | 66.66 | 0.34 | 0.58 | 0.66 |
14 | Ra2-A01 | 53 | 4 | 4 | 100.00 | 0.69 | 0.36 | 0.43 |
15 | Ra2-A11 | 55 | 5 | 5 | 100.00 | 0.73 | 0.35 | 0.41 |
16 | Ra2-C09 | 55 | 4 | 1 | 25.00 | 0.51 | 0.54 | 0.61 |
17 | Ra2-D04 | 54 | 3 | 1 | 33.33 | 0.53 | 0.45 | 0.54 |
18 | Ra2-E04 | 54 | 4 | 4 | 100.00 | 0.69 | 0.37 | 0.44 |
19 | Ra2-E11 | 54 | 7 | 7 | 100.00 | 0.67 | 0.45 | 0.49 |
20 | Ra2-E12 | 56 | 3 | 1 | 33.33 | 0.35 | 0.58 | 0.65 |
21 | Ra2-F11 | 54 | 6 | 6 | 100.00 | 0.77 | 0.32 | 0.36 |
22 | Ra2-G05 | 56 | 7 | 7 | 100.00 | 0.84 | 0.24 | 0.27 |
23 | Ra2-G08 | 54 | 5 | 5 | 100.00 | 0.76 | 0.31 | 0.36 |
24 | Ra2-G09 | 55 | 2 | 2 | 100.00 | 0.53 | 0.35 | 0.47 |
25 | Ra2-C11 | 56 | 4 | 4 | 100.00 | 0.71 | 0.34 | 0.41 |
26 | Ra3-H09 | 56 | 3 | 3 | 100.00 | 0.64 | 0.36 | 0.46 |
27 | Ra3-H10 | 54 | 6 | 6 | 100.00 | 0.75 | 0.34 | 0.38 |
28 | BrgMS60 | 54 | 3 | 1 | 33.33 | 0.66 | 0.35 | 0.45 |
29 | BrgMS63 | 54 | 3 | 1 | 33.33 | 0.67 | 0.34 | 0.44 |
30 | BrgMS68 | 55 | 5 | 1 | 20.00 | 0.60 | 0.37 | 0.48 |
31 | BrgMS70 | 54 | 4 | 4 | 100.00 | 0.72 | 0.33 | 0.40 |
32 | BrgMS75 | 54 | 3 | 2 | 66.66 | 0.63 | 0.37 | 0.47 |
33 | BrgMS309 | 54 | 2 | 1 | 50.00 | 0.50 | 0.38 | 0.50 |
34 | BrgMS316 | 53 | 3 | 1 | 33.33 | 0.66 | 0.35 | 0.45 |
35 | BrgMS329 | 54 | 2 | 1 | 50.00 | 0.66 | 0.26 | 0.34 |
36 | BrgMS334 | 57 | 3 | 2 | 66.66 | 0.63 | 0.37 | 0.47 |
37 | BrgMS337 | 53 | 5 | 1 | 20.00 | 0.70 | 0.38 | 0.44 |
38 | BrgMS338 | 56 | 3 | 3 | 100.00 | 0.70 | 0.30 | 0.37 |
39 | BrgMS344 | 53 | 4 | 3 | 75.00 | 0.73 | 0.32 | 0.39 |
40 | BrgMS372 | 55 | 4 | 3 | 75.00 | 0.70 | 0.35 | 0.42 |
41 | BrgMS388 | 56 | 4 | 4 | 100.00 | 0.71 | 0.34 | 0.41 |
42 | BrgMS397 | 54 | 4 | 1 | 25.00 | 0.69 | 0.36 | 0.43 |
43 | BrgMS399 | 55 | 4 | 4 | 100.00 | 0.68 | 0.37 | 0.44 |
44 | BrgMS409 | 55 | 3 | 3 | 100.00 | 0.67 | 0.34 | 0.44 |
45 | BrgMS412 | 53 | 5 | 5 | 100.00 | 0.74 | 0.34 | 0.40 |
46 | BrgMS418 | 56 | 2 | 1 | 50.00 | 0.50 | 0.38 | 0.50 |
47 | BrgMS421 | 54 | 3 | 2 | 66.66 | 0.60 | 0.39 | 0.48 |
48 | BrgMS422 | 55 | 3 | 2 | 66.66 | 0.59 | 0.41 | 0.50 |
49 | BrgMS429 | 54 | 3 | 2 | 66.66 | 0.41 | 0.54 | 0.61 |
50 | BrgMS430 | 55 | 3 | 2 | 66.66 | 0.65 | 0.36 | 0.45 |
51 | BrgMS432 | 54 | 3 | 1 | 33.33 | 0.66 | 0.35 | 0.44 |
52 | BrgMS521 | 55 | 3 | 3 | 100.00 | 0.67 | 0.34 | 0.44 |
53 | BrgMS590 | 54 | 4 | 1 | 25.00 | 0.72 | 0.33 | 0.40 |
54 | BrgMS638 | 53 | 3 | 3 | 100.00 | 0.34 | 0.59 | 0.67 |
55 | BrgMS710 | 54 | 2 | 2 | 100.00 | 0.52 | 0.36 | 0.48 |
56 | BrgMS713 | 55 | 3 | 1 | 33.33 | 0.34 | 0.59 | 0.67 |
57 | BrgMS751 | 54 | 2 | 1 | 50.00 | 0.50 | 0.38 | 0.50 |
58 | BrgMS776 | 55 | 4 | 4 | 100.00 | 0.74 | 0.31 | 0.38 |
59 | BrgMS778 | 54 | 3 | 1 | 33.33 | 0.67 | 0.35 | 0.44 |
60 | BrgMS780 | 55 | 3 | 3 | 100.00 | 0.65 | 0.36 | 0.46 |
61 | BrgMS782 | 54 | 4 | 4 | 100.00 | 0.69 | 0.36 | 0.43 |
62 | BrgMS783 | 54 | 2 | 1 | 50.00 | 0.51 | 0.37 | 0.49 |
63 | BrgMS787 | 54 | 6 | 5 | 83.33 | 0.66 | 0.46 | 0.51 |
64 | BrgMS794 | 53 | 2 | 2 | 100.00 | 0.51 | 0.37 | 0.49 |
65 | BrgMS799 | 54 | 2 | 1 | 50.00 | 0.50 | 0.38 | 0.50 |
66 | BrgMS801 | 56 | 4 | 4 | 100.00 | 0.69 | 0.36 | 0.43 |
67 | BrgMS802 | 54 | 2 | 1 | 50.00 | 0.50 | 0.38 | 0.50 |
68 | BrgMS848 | 54 | 2 | 1 | 50.00 | 0.50 | 0.38 | 0.50 |
69 | BrgMS961 | 55 | 3 | 2 | 66.66 | 0.57 | 0.42 | 0.51 |
70 | BrgMS1237 | 55 | 4 | 3 | 75.00 | 0.74 | 0.32 | 0.39 |
71 | BrGMS2766 | 51 | 4 | 1 | 25.00 | 0.74 | 0.32 | 0.39 |
72 | BrGMS2767 | 54 | 3 | 1 | 33.33 | 0.61 | 0.38 | 0.48 |
73 | BrgMS4508 | 55 | 4 | 4 | 100.00 | 0.71 | 0.34 | 0.41 |
74 | BrgMS4533 | 55 | 3 | 1 | 33.33 | 0.65 | 0.35 | 0.45 |
75 | Ni2A01 | 55 | 2 | 2 | 100.00 | 0.54 | 0.34 | 0.46 |
76 | Ni2A02 | 55 | 2 | 1 | 50.00 | 0.50 | 0.38 | 0.50 |
77 | Ni2A07 | 55 | 3 | 1 | 33.33 | 0.67 | 0.34 | 0.43 |
78 | Ni2A08 | 50 | 6 | 1 | 16.66 | 0.71 | 0.40 | 0.44 |
79 | Ni2A12 | 54 | 3 | 1 | 33.33 | 0.62 | 0.38 | 0.47 |
80 | Ni2B01 | 52 | 3 | 3 | 100.00 | 0.66 | 0.35 | 0.45 |
81 | Ni2B02 | 54 | 4 | 4 | 100.00 | 0.51 | 0.54 | 0.61 |
82 | Ni2B03 | 55 | 5 | 5 | 100.00 | 0.61 | 0.48 | 0.54 |
83 | Ni2B07 | 55 | 4 | 4 | 100.00 | 0.63 | 0.43 | 0.49 |
84 | Ni2B08 | 53 | 3 | 3 | 100.00 | 0.66 | 0.35 | 0.45 |
85 | Ni2C09 | 52 | 5 | 5 | 100.00 | 0.78 | 0.29 | 0.34 |
86 | Ni2D03 | 48 | 4 | 4 | 100.00 | 0.76 | 0.29 | 0.36 |
87 | Ni2D10 | 52 | 4 | 1 | 25.00 | 0.73 | 0.32 | 0.39 |
88 | Ni2E05 | 58 | 6 | 6 | 100.00 | 0.75 | 0.34 | 0.38 |
89 | Ni2F11 | 52 | 3 | 1 | 33.33 | 0.64 | 0.36 | 0.46 |
90 | Ni4A06 | 54 | 2 | 1 | 50.00 | 0.51 | 0.37 | 0.49 |
91 | Ni4A09 | 53 | 4 | 4 | 100.00 | 0.75 | 0.31 | 0.38 |
92 | Ni4B04 | 51 | 3 | 2 | 66.66 | 0.68 | 0.34 | 0.43 |
93 | Ni4C02 | 56 | 3 | 3 | 100.00 | 0.60 | 0.40 | 0.49 |
94 | Ni4C06 | 55 | 3 | 3 | 100.00 | 0.55 | 0.43 | 0.52 |
95 | Ni4C09 | 53 | 5 | 1 | 20.00 | 0.53 | 0.56 | 0.62 |
96 | Ni4C11 | 54 | 4 | 4 | 100.00 | 0.61 | 0.44 | 0.51 |
97 | Ni4F09 | 54 | 5 | 1 | 20.00 | 0.74 | 0.33 | 0.38 |
98 | Ni4F11 | 55 | 5 | 1 | 20.00 | 0.78 | 0.29 | 0.34 |
99 | Ni4G06 | 50 | 4 | 1 | 25.00 | 0.71 | 0.34 | 0.41 |
100 | Ni4G10 | 55 | 7 | 7 | 100.00 | 0.77 | 0.33 | 0.37 |
101 | nia-m091a | 52 | 3 | 1 | 33.33 | 0.65 | 0.36 | 0.46 |
102 | nia-m066a | 53 | 3 | 1 | 33.33 | 0.64 | 0.36 | 0.46 |
103 | SJ3838 | 51 | 5 | 5 | 100.00 | 0.79 | 0.28 | 0.33 |
104 | SJ4933 | 51 | 5 | 5 | 100.00 | 0.80 | 0.27 | 0.32 |
105 | SJ6846 | 52 | 5 | 5 | 100.00 | 0.76 | 0.31 | 0.36 |
106 | SJ3302RI | 52 | 2 | 1 | 50.00 | 0.50 | 0.38 | 0.50 |
107 | SJ03104 | 51 | 2 | 1 | 50.00 | 0.50 | 0.37 | 0.50 |
108 | SJ3627R | 52 | 4 | 4 | 100.00 | 0.71 | 0.34 | 0.41 |
109 | SB1672 | 48 | 5 | 5 | 100.00 | 0.69 | 0.39 | 0.44 |
110 | SB1752 | 53 | 4 | 4 | 100.00 | 0.61 | 0.44 | 0.51 |
111 | SB2131 | 50 | 2 | 1 | 50.00 | 0.50 | 0.38 | 0.50 |
112 | SA0306 | 51 | 5 | 5 | 100.00 | 0.52 | 0.56 | 0.61 |
113 | SB0372 | 53 | 3 | 3 | 100.00 | 0.62 | 0.38 | 0.48 |
114 | SB1935A | 51 | 5 | 3 | 60.00 | 0.72 | 0.35 | 0.40 |
115 | SJ8033 | 51 | 4 | 4 | 100.00 | 0.68 | 0.38 | 0.45 |
116 | SB0202I | 51 | 2 | 2 | 100.00 | 0.50 | 0.38 | 0.50 |
117 | SB3140 | 52 | 6 | 6 | 100.00 | 0.63 | 0.48 | 0.52 |
118 | SJ6842 | 53 | 4 | 1 | 25.00 | 0.57 | 0.48 | 0.55 |
119 | SB2556 | 52 | 3 | 1 | 33.33 | 0.63 | 0.37 | 0.46 |
120 | SB3872 | 51 | 2 | 2 | 100.00 | 0.50 | 0.38 | 0.50 |
121 | SJ0338 | 52 | 3 | 3 | 100.00 | 0.63 | 0.37 | 0.47 |
122 | SJ1505 | 49 | 2 | 1 | 50.00 | 0.51 | 0.37 | 0.49 |
123 | SJ3640I | 50 | 3 | 3 | 100.00 | 0.59 | 0.40 | 0.49 |
124 | SJ39119I | 51 | 4 | 4 | 100.00 | 0.68 | 0.37 | 0.44 |
125 | SJ13133 | 51 | 4 | 3 | 75.00 | 0.62 | 0.42 | 0.49 |
126 | SJ1536 | 51 | 5 | 5 | 100.00 | 0.68 | 0.40 | 0.46 |
127 | SB1937 | 50 | 3 | 2 | 66.66 | 0.64 | 0.36 | 0.46 |
128 | SJ4633 | 47 | 2 | 2 | 100.00 | 0.50 | 0.38 | 0.50 |
129 | SB1728 | 52 | 3 | 3 | 100.00 | 0.62 | 0.38 | 0.47 |
130 | SJ34121 | 51 | 4 | 3 | 75.00 | 0.66 | 0.39 | 0.46 |
131 | SJ1668I | 50 | 2 | 1 | 50.00 | 0.50 | 0.38 | 0.50 |
132 | SB3751 | 51 | 2 | 1 | 50.00 | 0.50 | 0.37 | 0.50 |
133 | cnu_m587a | 52 | 3 | 3 | 100.00 | 0.65 | 0.35 | 0.45 |
134 | cnu_m593a | 52 | 2 | 1 | 50.00 | 0.50 | 0.38 | 0.50 |
135 | cnu_m596a | 53 | 4 | 1 | 25.00 | 0.72 | 0.33 | 0.40 |
136 | cnu_m597a | 54 | 4 | 4 | 100.00 | 0.72 | 0.33 | 0.40 |
137 | cnu_m600a | 51 | 4 | 1 | 25.00 | 0.75 | 0.30 | 0.37 |
138 | cnu_m602a | 56 | 3 | 1 | 33.33 | 0.65 | 0.35 | 0.45 |
139 | cnu_m604a | 53 | 6 | 1 | 16.66 | 0.51 | 0.61 | 0.66 |
140 | cnu_m611a | 55 | 2 | 2 | 100.00 | 0.51 | 0.37 | 0.49 |
141 | cnu_m626a | 54 | 5 | 5 | 100.00 | 0.73 | 0.34 | 0.40 |
142 | sORA43 | 55 | 4 | 1 | 25.00 | 0.70 | 0.35 | 0.42 |
143 | Ol09A01 | 54 | 5 | 5 | 100.00 | 0.79 | 0.28 | 0.33 |
144 | Ol10A11 | 55 | 3 | 1 | 33.33 | 0.34 | 0.59 | 0.66 |
145 | Ol10B01 | 54 | 4 | 1 | 25.00 | 0.45 | 0.58 | 0.64 |
146 | Ol10B11 | 52 | 7 | 7 | 100.00 | 0.79 | 0.31 | 0.34 |
147 | KBRH138G23 | 52 | 3 | 3 | 100.00 | 0.67 | 0.34 | 0.44 |
148 | KBRH139B23 | 52 | 3 | 3 | 100.00 | 0.66 | 0.35 | 0.45 |
149 | E018 | 55 | 3 | 3 | 100.00 | 0.66 | 0.35 | 0.45 |
150 | GOL2 | 56 | 3 | 2 | 66.66 | 0.38 | 0.56 | 0.63 |
151 | GOL3 | 55 | 3 | 3 | 100.00 | 0.34 | 0.59 | 0.66 |
152 | EJU1 | 53 | 2 | 1 | 50.00 | 0.50 | 0.38 | 0.50 |
153 | EJU3 | 52 | 4 | 1 | 25.00 | 0.73 | 0.32 | 0.39 |
154 | EJU5 | 55 | 3 | 1 | 33.33 | 0.57 | 0.41 | 0.51 |
155 | ENA2 | 55 | 5 | 5 | 100.00 | 0.60 | 0.50 | 0.56 |
156 | ENA20 | 54 | 4 | 4 | 100.00 | 0.72 | 0.33 | 0.40 |
157 | ENA28 | 54 | 2 | 1 | 50.00 | 0.51 | 0.37 | 0.49 |
158 | ENA9 | 54 | 3 | 1 | 33.33 | 0.45 | 0.50 | 0.59 |
159 | MB4 | 46 | 2 | 2 | 100.00 | 0.51 | 0.37 | 0.49 |
Mean | 3.61 | 0.62 | 0.39 | 0.47 |