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. 2022 Dec 1;299(1):102759. doi: 10.1016/j.jbc.2022.102759

Figure 7.

Figure 7

TrmK recognizes the U8 and G18 in other tRNA species.A–C, secondary structures of (A) tRNASer_UGA, (B) tRNAGlu_UCC, and (C) tRNAGly_UCC are drawn. D, the methyl group acceptance activities in tRNASer_UGA WT, U8A, G18U, and G45cC were measured by a gel assay. The reaction was performed at 60 °C for 10 min. E, the methyl group acceptance activities in WT, U8A, A13U, and G18U for tRNAGlu_UUC and WT, U13A, U13A_U8A, and U13A_G18U for tRNAGly_UCC were measured. The reaction was performed at 60 °C for 10 min. F, the methyl group acceptance activity in the tRNAGly_UCCs was measured. The reaction was performed at 50 °C or 45 °C for 30 min. The contrast of the gel image was adjusted due to the slight methyl group acceptance activity in tRNAGly as compared with that in tRNAGlu.