FIG. 3.

Gene expression of cytokines (A) or IL-12Rβ subunits (B) in the enriched CD4⁺ T cells from IL-2/IL-15Rβ- or IL-2-deficient mice or wild-type mice on day 6 after i.p. infection with serovar Choleraesuis (2 × 10⁶ CFU). Total RNA was extracted from the magnetic beads cell sorter column-sorted CD4⁺ T cells by an acid guanidinium thiocyanate-phenol-chloroform method and reverse transcribed, and the cDNA was amplified using specific primers. After amplification, the PCR products were resolved on 1.8% agarose gels and Southern blotted using cytokine-specific internal oligonucleotide probes. After hybridization, the membrane was exposed to X-ray film. The data are representative of three independent experiments using pooled cells from three to five mice of each group.