FIG. 4.
Cytokine production by PEC from IL-2/IL-15Rβ- or IL-2-deficient mice or wild-type mice on day 6 after i.p. infection with serovar Choleraesuis (2 × 106 CFU) in response to HKS. The enriched T cells (5 × 105) were cultured with HKS in the presence of mitomycin C-treated spleen cells (5 × 105) from uninfected C57BL/6 mice for 2 days at 37°C. Thereafter, the supernatants were collected, and cytokine activity was determined by an enzyme-linked immunosorbent assay. The data are representative of three independent experiments using pooled cells from three to five deficient mice and are shown as the means of triplicate determinations ± SDs. ND, not detected.