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. 2022 Dec 15;67:101659. doi: 10.1016/j.molmet.2022.101659

Figure 4.

Figure 4

SCD1 regulates the methylation status of CpG sites in Pdx1 and MafA gene promoters in pancreatic INS-1E cells. (A, B) mRNA levels of Pdx1(A) and MafA(B) genes in INS-1E cells after SCD1 inhibition (SCD1i) and/or subsequently co-treated with palmitate (16:0). ∗p < 0.05, vs. vehicle; ˆp < 0.05, vs. SCD1i. The data are expressed as the mean ± SD from three independent experiments. (C–F) Bisulfite sequencing analysis of Pdx1 and MafA promoter regions (−1000 bp to 0 bp from transcription start site): methylation status and distribution of 5-methylcytosines alongside Pdx1(C) and MafA(D) promoter sequences, rate of cytosine DNA methylation in Pdx1 and MafA promoters in INS-1E cells after SCD1 inhibition (SCD1i) and/or palmitate (16:0) treatment. Blue boxes indicate CpG islands (regions enriched in CG dinucleotides) in the DNA sequence. n = 3. TSS, transcription start site.