Figure 4.

The rats were randomly allocated to Sham (n = 15), ECPR (n = 20), and SHCP combined ECPR (CP-ECPR) (n = 20) groups. Brain injury serum markers, pathomorphologic changes in the hippocampus, RNA sequencing, microglia activation, and inflammatory cytokines in brain tissues and serum were detected. The main results showed that SHCP rapidly reduced the brain-targeted temperature and brain injury serum biomarker levels. SHCP could serve as a novel approach to alleviate neuronal damage in ECPR rats. MAP, Mean arterial pressure; ECPR, extracorporeal cardiopulmonary resuscitation; SHCP, selective hypothermic cerebral perfusion; CP-ECPR, selective hypothermic cerebral perfusion combined ECPR; RNA, ribonucleic acid; S100β, S-100β protein.