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. 2022 May 3;14(6):615–628. doi: 10.1159/000524434

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Copyright © 2022 by The Author(s). Published by S. Karger AG, Basel

This article is licensed under the Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC). Usage and distribution for commercial purposes requires written permission.

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Fig. 1 — Sample IHC images (×20 magnification) of double staining for CD68 (red) and CD163 (brown) and single staining for MARCO (brown). Double positive (CD68⁺/CD163⁺) TAMs are indicated by black arrowheads, single positive (CD68⁺/CD163⁻) TAMs are indicated by white arrowheads, and MARCO⁺ TAMs are indicated by red arrowheads. a Predominance of CD68⁺/CD163⁺ TAMs, mainly infiltrating in the stroma and periphery of the TN. b Predominance of CD68⁺/CD163⁻ TAMs, mainly infiltrating in the stroma and periphery of the TN. c Predominance of CD68⁺/CD163⁺ TAMs with infiltration into TN. d Predominance of CD68⁺/CD163⁻ TAMs with infiltration into TN. e, f MARCO⁺ TAMs, mainly located in the tumor stroma.