Figure 3.

Glutamine (Gln) supplementation augments macrophage M2 polarization in IL-4-treated J774A.1 cells. (a) J774A.1 cells were treated with 50 ng/ml IL-4 alone or together with 2 mM Gln for 24 h. mRNA expression of Arg1, Fizz1 and Ym1 was detected by RT-PCR. (b) F4/80⁺/CD11b⁺/CD206⁺ cells were detected by flow cytometry. (c) Protein expression of Arg1 and CD206 was detected by western blot. ^**p < 0.01. N.S. no significance. n = 3. IL interleukin