Figure 5.

Glutamine (Gln) promotes PDH activity without upregulating its expression or affecting its protein phosphorylation. (a,b) J774A.1 cells were transfected with PDHA1 siRNA (siPDHA1) for 48 h. Then cells were treated with 50 ng/ml IL-4 alone or together with 2 mM Gln for 24 h. mRNA (a) and protein (b) expression of PDHA1 were detected by RT-PCR and western blot, respectively. (c) BMDMs were treated with 50 ng/ml IL-4 alone or together with 2 mM Gln for 24 h. The phosphorylation of PDHA1 (p-PDHA1) was detected by western blot. ^**p < 0.01. N.S. no significance. n = 3. BMDMs bone marrow derived macrophages, IL interleukin