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. 2022 Dec 16;16:917251. doi: 10.3389/fncir.2022.917251

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Copyright © 2022 Lu, Xu, Hayworth, Pang, Shinomiya, Plaza, Scheffer, Rubin, Hess, Rivlin and Meinertzhagen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Quality of the cut surface of hot-knife slice. (A) Entire FIB-SEM image of the medulla neuropile of a 20 μm thick hot-knife slice shown in (B). (B) Light micrograph of 20 μm slice of optic lobe. (C) Enlarged area of one side of the cut surface, shown enclosed in yellow box in (A). Arrows in (A) show the smoothness of the cut surface of the slice. Arrowhead in (C,D) shows selected synaptic profile. Arrows in (C,D) show the surface of the hot-knife slice, smooth (C) but occasionally rough (D). Scale bars: 10 μm (A); 100 μm (B); 1 μm (C,D).