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. 2022 Dec 30;8:104. doi: 10.1038/s41522-022-00368-y

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Female and male mice were immunized with an HIV T-cell vaccine (three DNA.HTI primes followed by a ChAd.HTI and MVA.HTI booster; n = 20) or a mock vaccine (PBS; n = 20). Antibiotic conditioning followed by fecal microbiota transfer was performed prior to vaccination to homogenize the intestinal content across all animals. Collection of samples, including stool, luminal content, serum and spleen was completed at the indicated time points. Abbreviations: FMT fecal microbiota transfer, Atb Antibiotic, PBS phosphate-buffered saline. The Figure was partly generated using Servier Medical Art, provided by Servier, licensed under a Creative Commons Attribution 3.0 unported license.