Table 1.
Baseline characteristics at baseline (0 week, pre-vaccination).
| Group | Mouse Line | Age (weeks) | Cage | N mice | Sex | Weight (g ± SD)11 | Sequencing Batch | Microbiota composition2 |
|---|---|---|---|---|---|---|---|---|
| Mock | C57BL/6JOlaHsd | 9 | C19 | 5 | F | 19.3 ± 0.9 | B1 | Lachnospiraceae NK4A136 group (10.8%), Bifidobacterium (5.7%), Bacteroides (5.3%), Lactobacillus (3.4%), Turicibacter (1.7%) |
| Mock | C57BL/6JOlaHsd | 9 | C20 | 5 | M | 25.4 ± 1.4 | B1 | Bacteroides (11.4%), Lachnospiraceae NK4A136 group (7.9%), Bifidobacterium (6.2%), Alistipes (2.5%), Anaerostipes (1.7%) |
| Mock | C57BL/6JOlaHsd | 9 | C31 | 5 | F | 19.4 ± 1 | B2 | Bacteroides (16.3%), Lachnospiraceae NK4A136 group (12.2%), Bifidobacterium (5.1%), Lactobacillus (3.1%), Mucispirillum (2.7%) |
| Mock | C57BL/6JOlaHsd | 9 | C32 | 5 | M | 25.3 ± 1.3 | B2 | Bacteroides (19.2%), Bifidobacterium (10.4%), Lachnospiraceae NK4A136 group (10.1%), Alistipes (3.2%), Lactobacillus (2.1%) |
| Immunized | C57BL/6JOlaHsd | 9 | C21 | 5 | F | 19.5 ± 0.9 | B1 | Bacteroides (11.7%), Lachnospiraceae NK4A136 group (11.7%), Bifidobacterium (6.6%), Alistipes (1.8%), Lactobacillus (3.9%) |
| Immunized | C57BL/6JOlaHsd | 9 | C22 | 5 | M | 25.6 ± 2 | B1 | Lachnospiraceae NK4A136 group (14.1%), Bifidobacterium (7.4%), Bacteroides (6.7%), Alistipes (3.2%), Lactobacillus (1.6%), |
| Immunized | C57BL/6JOlaHsd | 9 | C29 | 5 | F | 17.2 ± 0.6 | B2 | Bacteroides (14.1%), Bifidobacterium (12.9%), Lachnospiraceae NK4A136 group (8.5%), Lactobacillus (3.4%), Faecalibaculum (2.1%) |
1mean grams and sd.
2percentage of top 5 most abundant taxa; complete list of taxa is provided in Supplementary Table 3.