Table 3. Relative Amounts of the Different Linkage Types Formed per HCAgm after 120 min of Incubation with HRP on a Small Scalea.
| relative amounts (± st. dev.) |
|||
|---|---|---|---|
| CouAgm | FerAgm | SinAgm | |
| 4-O-7′/3-8′-linkage | 62.4 (± 1.7) | 57.9 (± 4.1) | 0.0 (± 0.0) |
| 2-7′/8-8′-linkage | 0.0 (± 0.0) | 18.3 (± 0.7) | 60.6 (± 0.7) |
| 8-8′/9-N-7′-linkage | 7.3 (± 0.6) | 2.0 (± 0.3) | 25.4 (± 1.5) |
| 4-O-8′-linkage | 4.9 (± 0.0) | 14.4 (± 0.4) | 0.0 (± 0.0) |
| 8-8′-linkage | 14.0 (± 0.3) | 4.8 (± 0.4) | 14.0 (± 0.2) |
| unidentified | 11.3 (± 0.6) | 2.6 (± 0.5) | 0.0 (± 0.0) |
Percentages are determined based on the relative UV320 peak area for all dimers. Standard deviations (st. dev.) are based on triplicates.