Skip to main content
. 2022 Dec 30;11(1):e01047. doi: 10.1002/prp2.1047

FIGURE 1.

FIGURE 1

Transient exposure to EGF and TGF‐α promoted primary resistance to ALK/c‐Met TKI in H3122 and EBC‐1 cells. (A) Summary of results from H3122 and EBC‐1 cells treated with eight widespread growth factors in the presence of TAE684 (1 μM) or SGX‐523 (1 μM). CR, complete resistance; PR, partial resistance; NR, no rescue. (B, C) H3122 cells (B) or EBC‐1 cells (C) were treated with growth factors in the presence of TAE684 (1 μM) or SGX‐523 (1 μM) for 72 h and cell viability was assessed by MTT assay. Data are shown as mean ± SD from three independent experiments. **p < .01, ***p < .001. (D, E) H3122 cells (D) or EBC‐1 cells (E) were treated with TAE684 (1 μM) or SGX‐523 (1 μM) for 12 h and incubated with growth factors for additional 30 min followed by western blotting analysis with indicated antibodies.