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. 2022 Dec 30;11(1):e01047. doi: 10.1002/prp2.1047

FIGURE 2.

FIGURE 2

Increased autocrine EGF and TGF‐α activated EGFR pathway in acquired resistant H3122/TR and EBC‐1/SR cells. (A, B) Sensitive (H3122, EBC‐1) and resistant (H3122/TR, EBC‐1/SR) cells were treated with different concentrations of TAE684 (A) or SGX‐523 (B) for 72 h and cell viability was assessed by MTT assay. Data are shown as mean ± SD from three independent experiments. (C, D) Sensitive and resistant cells were treated with TAE684 (1 μM) (C) or SGX‐523 (1 μM) (D) for 3 h and the whole cell lysates was subjected to western blotting with indicated antibodies. (E, F) ELISA of HGF, EGF and TGF‐α secretion by sensitive or resistant cells. Data are shown as mean ± SD from three independent experiments. *p < .05, **p < .01, ***p < .001. (G, H) Western blotting analysis of protein phosphorylation in sensitive and resistant cells with indicated antibodies. Results of three independent experiments are shown.