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. 2022 Dec 14;8(12):1695–1703. doi: 10.1021/acscentsci.2c00998

Figure 1.

Figure 1

Identification and construction of cell permeant Nb ligands for BCL11A. (A) Disorder probability of BCL11A revealing predicted order in the ZnF domains (0–6) and the sequence divergence from BCL11B in the extended ZnF23 region. The ZnF23 domains of the paralogs are 93.2% identical and 96.6% similar; in the exZnF23 fragment, the sequence identity and similarity are 69.3% and 78.1%, respectively. (B) Flowchart summarizing Nb selection. (C) SDS-PAGE analysis showing that Nb 2D9 and 2D9_W108L interact with exZnF23 of BCL11A (exZnF23A) but not ZnF23 and exZnF23 of BCL11B (exZnF23B).