Fig. 2.

Illustration of the four scRNA-seq protocols applied in this study. A NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina (NEB®) followed by NEBNext® uracil excision based (UEB) Final Library Preparation KIT. B SMART-seq® High-Throughput (HT) kit (Takara Bio Inc.) followed by final library preparation using Nextera XT Library preparation kit (Illumina, USA). C Genome & transcriptome sequencing (G&T-seq) followed by final library preparation using Nextera XT Library preparation kit (Illumina, USA). D SMART-seq3 (SS3) followed by final library preparation using Nextera XT Library preparation kit (Illumina, USA) TN5