Table 2.

Table constituting the number of cells processed and cells remaining post QC filtering

Protocol	NEB®	Takara®	G&T	SS3
Cells Processed	14	13	13	14
Cells Post Filtering	11	11	11	11
Price per SC (12rxn) / per 96rxn	46 €a / 4.024 €a	75 €a / 5.955 €a	12 €a/ 1.152 €a	10 €a/ 960 €a
Full Length	✓	✓	✓	✓
LNA	✗	✓	✓	✗
UMI	✗	✗	✗	✓

All prices are per single cell using the smallest commercially available format of 12 cell reactions (rxn), as quoted medio 2020. All tested protocols feature full-length sequencing of transcripts. Takara® and G&T protocol genes a TSO with a locked Nucleic Acid (LNA), whereas NEB® and SS3 does not. Only SS3 features UMI’s

^aPrice includes reagents for reverse transcription, PCR amplification, and final library preparation for all protocols. Price for protocols Takara, G&T & SS3 include price for final library preparation at 8 € per cell for ¼ Nextera XT DNA library preparation (Cat. Nr: FC-131–1096) according to recommendation by kit manufacturer (Takara Bio Inc.) and protocol (G&T) [16]