Table 3.
Irritation score and classification of damage to the chorioallantoic membrane (n = 4/group).
| Sample | Irritation score (IS) | Classification |
|---|---|---|
| NaOH 0,1 M | 13,31 ± 0,26a | Severe irritant |
| Sodium lauryl sulfate 1% m/v | 10,65 ± 0,23a | Severe irritant |
| NaCl 0,9% m/v | 0,00 ± 0,00b | Non-irritant |
| MCT | 0,00 ± 0,00b | Non-irritant |
| D-limoneno pure | 6,24 ± 0,32a,b | Moderate irritant |
| Pure Lime EO | 8,57 ± 0,24a,b | Moderate irritant |
| Pure Orange EO | 6,95 ± 0,0,29a,b | Moderate irritant |
| d-limonene 10 mg/mL | 0,00 ± 0,00b | Non-irritant |
| Lime EO 10 mg/mL | 0,00 ± 0,00b | Non-irritant |
| Lime EO 10 mg/mL | 0,00 ± 0,00b | Non-irritant |
Statistical analysis: ANOVA, post hoc Tukey test (p<0,05): a - different from negative control (NaCl 0,9% m/v); b – different from positive control (NaOH 0,1 M and sodium lauryl sulfate 1% m/v). MCT: medium-chain triglycerides; EO: essential oil.