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. 2022 Aug 5;189(2):186–202. doi: 10.1093/toxsci/kfac080

Figure 4.

Figure 4.

Regulation of nicotine-induced SLBP depletion by CDK1/2. A and B, Inhibition of CDKs attenuates nicotine-induced downregulation of SLBP. BEAS-2B cells were treated with or without nicotine for 24 h in the presence or absence of roscovitine, an CDK inhibitor, and subjected to Western blot (A). The band intensities were quantified and presented as bar graphs to show relative quantifications of SLBP (B). GAPDH was used as an internal control. C–F, Knockdown of CDK1 by siRNA attenuates nicotine-induced downregulation of SLBP but not phosphorylation of AKT at S473. BEAS-2B cells were transiently transfected with control siRNA or CDK1 siRNA and then treated with or without nicotine for 24 h followed by Western blot analysis with indicated antibodies (C). The band intensities were quantified and presented as bar graphs to show relative quantifications of CDK1 (D), p-AKTS473/AKT (E), and SLBP (F). G–J, Knockdown of CDK2 by siRNA attenuates nicotine-induced downregulation of SLBP and phosphorylation of AKT at S473. BEAS-2B cells were transiently transfected with control siRNA or CDK2 siRNA and then treated with or without nicotine for 24 h followed by Western blot analysis with indicated antibodies (G). The band intensities were quantified and presented as bar graphs to show relative quantifications of CDK2 (H), p-AKTS473/AKT (I), and SLBP (J). Untreated controls in lane 1 were used as references, which were set to 1. The data shown are the mean ± SD (n = 3). *p < .05 versus untreated control group.