Figure 6.

Nicotine-induced activation of AKT, CDK1, and CK2 is α7-nAChR dependent. A, Knockdown of α7-nAChR by siRNA attenuates nicotine-induced activation of AKT, CDK1, and CK2. BEAS-2B cells were transiently transfected with control siRNA or α7-nAChR siRNA and then treated with or without nicotine for 24 h followed by Western blot analysis with indicated antibodies. B–F, The band intensities from (A) were quantified and presented as bar graphs to show relative quantifications of α7-nAChR (B), p-AKTS473/AKT (C), CDK1 (D), CDK2 (E), and CK2 (F). GAPDH was used as an internal control. The controls in lane 1 were used as references. The data shown are the mean ± SD (n = 3). *p < .05 versus control group.