FIG. 5.

Binding of nuclear proteins from H. pylori-infected gastric epithelial cell lines to the A1 and A2 probes derived from the MCP-1 distal enhancer. (A) NF-κB activation in several gastric epithelial cell lines treated for 1 h with H. pylori (ATCC 49503). Nuclear extracts were prepared from the indicated cell lines and incubated with radiolabeled A1 (lanes 1 to 10) and A2 (lanes 11 to 20) probes. (B) Time course of NF-κB activation in MKN45 cells infected with H. pylori, evaluated by EMSA. (C) cag PAI products of H. pylori are required for induction of NF-κB binding activity in MKN45 cells. Nuclear extracts from unstimulated MKN45 cells (lanes 1 and 6) and cells cocultured with the indicated H. pylori strains (lanes 2 to 5 and 7 to 10) were analyzed for NF-κB.