FIG. 4.
Effect of the antioxidants NAC and GSH on monocyte ROI production triggered by phagocytosis of S. aureus (monocyte-to-bacterium ratio of 1:20). ROIs were detected by chemiluminescence and by flow cytometry using the fluorogenic probes HE or DHR 123. Integrals of total ROI produced by 105 monocytes (Mø) during 30-min chemiluminescence measurement (A) and histograms of red (HE; B) and green (DHR 123; C) fluorescence intensity corresponding to O2− and H2O2 production, respectively, are shown. Dotted lines, control monocytes; solid black lines, monocytes stimulated with S. aureus; solid grey lines, monocytes preincubated with 25 mM NAC and stimulated with S. aureus; dotted grey lines, monocytes preincubated with 25 mM GSH and stimulated with S. aureus. Results from one of three experiments performed are shown.