TABLE 2.
sFasL in supernatants of monocytes cultured with and without S. aureusa
| Expt | sFasL (pg/ml)
|
||
|---|---|---|---|
| Control | S. aureus | Zymosan | |
| 1 | 102.1 | ND | 259.6 |
| 2 | 0 | 487.0 | 197.0 |
| 3 | 57.3 | 204.2, 117.7b | ND |
| 4 | |||
| −DPI | 0 | 100.0 | ND |
| +DPI | 0 | 0 | ND |
| 5 | |||
| −DPI | 0 | 33.6 | ND |
| +DPI | 0 | 0 | ND |
| 6 | |||
| −DPI | 36.0 | 64.6 | 51.0 |
| +DPI | 0 | 43.1 | 39.2 |
| 7 | |||
| −DPI | 0 | 18.5 | 2.5 |
| +DPI | 0 | 1.4 | ND |
a
Monocytes (5 × 106/ml) were incubated with either opsonized S. aureus (ratio of 1:20) or zymosan particles (0.5 mg/ml) for 1 h (experiments 6 and 7) or 6 h (experiments 1 to 5). Thereafter, culture supernatants were collected and concentrated, and sFasL was detected by ELISA. ND, not determined.
b
Heat-killed S. aureus was used.