FIG. 2.

Effect of WT and various EPEC mutant strains tyrosine activation of p46 and p52 Shc isoforms. Lysates from uninfected T84 cells (control) and cells infected for 1 h with WT strain E2348/69 or mutant strains CVD206 and CVD452 were immunoprecipitated with anti-Shc antibody. The immunocomplex was subjected to electrophoresis on an SDS–9% polyacrylamide gel and analyzed by immunoblotting using antiphosphotyrosine antibodies. The positive control was obtained from cells treated with EGF (10 nM, 15 min). Identical results were obtained at least three times.