FIG. 4.

Activation of p38 MAP kinases in E2348/69-infected cells. (A) T84 cells were lysed at different times after infection. Samples were resolved by electrophoresis on an SDS–9% polyacrylamide gel and analyzed by immunoblotting using anti-phosphorylated p38 antibodies. (B) T84 cells were infected for 1 h with the WT strain E2348/69 and EPEC mutant strains CVD206 and CVD452. Samples were resolved as described above. The control lane corresponds to uninfected T84 cells. The positive control was obtained from cells treated with EGF (10 nM, 15 min). Identical results were obtained at least three times.