FIG. 6.

MAP kinase inhibitors have no effect on the EPEC-induced decrease of TER in T84 monolayers. Bacteria (100/cell) were added to the apical surface of T84 cells. Resistance decreased in cells infected with the WT strain E2348/69 (■) compared to control monolayers (⧫). When indicated, the inhibitors PD98059 (●) and SB203580 (▴), alone or together (▾), were added to the cell medium 90 min prior to infection, and the inhibitors were present during the infection. In cells treated with inhibitors, TER remained comparable to that in EPEC-infected cells. Each point represents the mean value obtained from at least six individual T84 monolayers. Error bars show the standard deviation. ∗ denotes significantly different from control monolayers (P < 0.02) when compared by the Bonferroni/Dunn tests.