TABLE 1.
Bacterial strains used in this study
| Strain | Characteristics (reference) | T84 cell response to infection
|
||
|---|---|---|---|---|
| Barrier function (% change in resistance vs control)a | A/E lesionb | IL-8 (ng/ml)c | ||
| E2348/69 | Parental EPEC, Adh+ Inv+ (26) | −78 ± 0.2 | + | 54 ± 6.8 |
| CVD206 | E2348/69 ΔeaeA, Adh+ intimate Adh− Inv− (10) | −12 ± 0.1 | − | 2.8 ± 0.82 |
| CVD452 | E2348/69 ΔescN::aphA-3, signal transduction affected (20) | +6 ± 0.3 | − | 1.9 ± 0.49 |
a
TER was monitored on filter-grown T84 cells as described in Materials and Methods. Values presented correspond to resistance measured after 6 h of infection.
b
Estimated after 3 h of infection by staining with FITC-phalloidin.
c
Estimated in cell supernatant from 6-h-infected cells by enzyme-linked immunosorbent assay as described in Materials and Methods.