Vector competence of wAlbB-infected Ae. aegypti, on either the Mexican or Singaporean genetic background, for ZIKV. The infection rates and titers of ZIKV LP0210Y17 strain, an Asian lineage (a, b), and SG(EHI)ZIKV/33164Y17, a South American lineage (c, d), in the midguts (a, c) and salivary glands (b, d) at 7 and 14 DPI. The virus titers were calculated as log10TCID50/ml. W+, wAlbB-infected mosquitoes on either the Mexican genetic background (WBM) or Singaporean genetic background (WBSG); W-, wild-type Ae. aegypti (Wolbachia free) on either the Mexican genetic background (AFM) or Singaporean genetic background (WTSG). Lines and error bars denote medians ± 95% CIs of viral titers with nonzero values, and each point represents an individual midgut/salivary gland. Significant differences in viral titers and infection rate (prevalence) were determined using Mann–Whitney test and two-sided Fisher’s exact test, respectively. *P < 0.05, **P < 0.01, ****P < 0.0001.