Table 1.
Single-generation tests for SSA-based transgene elimination induced by the I-SceI-expressing trigger strains (G4), Nos-I-SceI and PUb-I-SceI.
| F2 Larval screeninga | |||||||
|---|---|---|---|---|---|---|---|
| Parental cross (♂30 × ♀100) | Lineage of the SSA triggerb | I-SceI inherited to F1 adults | c # Total | # WGR No DSB (kmoRG/Δ4) | # WG NHEJ (kmoG/Δ4) | # W kmo-null (kmoΔ4/Δ4) | # Blk SSA (kmo+/Δ4) |
| Nos-I-SceI x kmoRG | F0♂-F1♂ | + | 7,500 | 4,122 | 23 (0.56%) | 3,315 | 40 (0.97%) |
| – | 7,252 | 3,609 | 1 (0.03%) | 3,642 | 0 | ||
| F0♂-F1♀ | + | 2,588 | 1,608 | 9 (0.56%) | 957 | 14 (0.87%) | |
| – | 4,867 | 2,410 | 0 | 2,457 | 0 | ||
| F0♀-F1♂ | + | 7,828 | 4,268 | 74 (1.73%) | 3,380 | 106 (2.48%) | |
| – | 7,477 | 3,839 | 93 (2.42%) | 3,528 | 17 (0.44%) | ||
| F0♀-F1♀ | + | 4,676 | 2,722 | 36 (1.32%) | 1,835 | 83 (3.05%) | |
| – | 5,749 | 3,077 | 10 (0.32%) | 2,652 | 10 (0.32%) | ||
| PUb-I-SceI x kmoRG | F0♂-F1♂ | + | 494 | 310 | 0 | 184 | 0 |
| – | 1,211 | 617 | 0 | 594 | 0 | ||
| F0♂-F1♀ | + | 1,370 | 823 | 0 | 547 | 0 | |
| – | 2,904 | 1,473 | 0 | 1431 | 0 | ||
| F0♀-F1♂ | + | 1,850 | 1,133 | 0 | 717 | 0 | |
| – | 1,302 | 684 | 0 | 618 | 0 | ||
| F0♀-F1♀ | + | 1,450 | 820 | 0 | 630 | 0 | |
| – | 1,277 | 660 | 0 | 617 | 0 | ||
a
W, white eye; Blk, black eye; G, EGFP; R, DsRED; and B, BFP.
b
nos-driven germline cell-specific or PUb-driven ectopic expression of the HE, I-SceI.
c
The Mariner Mos1-based transgenic I-SceI allele, which is inherited from the parental SSA trigger strain, provides the eye-specific BFP fluorescence.