FIG. 4.

The effect of Z-VAD-FMK on the expression of iNOS protein in LPS-stimulated RAW cells. RAW cells were pretreated with Z-VAD-FMK (100 μM) for 30 min. Z-VAD-FMK-pretreated or untreated control cells were incubated with LPS (1 μg/ml) for 8 h. The cells were lysed, and the lysates were analyzed by immunoblotting using an anti-iNOS antibody.