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The effect of Z-VAD-FMK on the phosphorylation of Erk1/2, p38, and JNK/SAPK MAP kinases in LPS-stimulated RAW cells. RAW cells were pretreated with Z-VAD-FMK (100 μm) for 30 min, followed by treatment with LPS (1 μg/ml) for 30 min. The cells were lysed, and the lysates were analyzed by immunoblotting using antibodies to (A) phospho -Erk1/2, (B) phospho-p38, or (C) phospho-JNK/SAPK. The antibody against Erk1/2, p38, or JNK/SAPK was also used.
